Catherine O'Hara (Waiting for Guffman, Best in Show, Six Feet Under) in Disney's Tall Tale (1995)
Doris Day in Calamity Jane (1953)
It would be remiss to feature Doris Day without mentioning her famously queer song "Secret Love" from the Calamity Jane film. The film came out during the Hollywood code when homosexuality, lesbian and the like were dirty words. Lesbians clung to Doris' performance as Calamity pining for her secret love, as cited in The Celluloid Closet.